Extraction, Partial Purification and Characterization of Bromelain from Pineapple (Ananas Comosus) Crown, Core and Peel Waste
نویسندگان
چکیده
HIGHLIGHTS Bromelain was extracted from crown, core, and peel waste of pineapple. Ammonium sulphate (NH4)2SO4 precipitation method used as a simple cost-effective approach for partial purification bromelain. A 4.34, 2.75 2.59-fold achieved core bromelain samples respectively. Enzyme activity recovery 170.41 %, 192.37 % 337.59 bromelain,
منابع مشابه
Studies of stability and characterization this enzyme bromelain in pineapple (Ananas comosus)
Background Bromelain is the generic name given to the set of derived endopeptidases belonging to members of the Bromeliaceae family, which belongs to the pineapple (Ananas comosus), being able to break the peptide bond, separating proteins and amino acids [1]. Bromelain possesses a wide range of therapeutic benefit as property of facilitating digestion of proteins, meat softening, ability to fa...
متن کاملPurification, characterization, and antifungal activity of chitinases from pineapple (Ananas comosus) leaf.
Three chitinases, designated pineapple leaf chitinase (PL Chi)-A, -B, and -C were purified from the leaves of pineapple (Ananas comosus) using chitin affinity column chromatography followed by several column chromatographies. PL Chi-A is a class III chitinase having a molecular mass of 25 kDa and an isoelectric point of 4.4. PL Chi-B and -C are class I chitinases having molecular masses of 33 k...
متن کاملIsolation and Purification of Bromelain from Waste Peel of Pineapple for Therapeutic Application
The aim of this work was to isolate and purify bromelain extracted from the pineapple peel by ammonium sulfate precipitation (40-80%), followed by desalting and freeze-drying with a 75% activity recovery and 2.2 fold increased specific activity. Ion exchange chromatography on DEAE-Sepharose was able to separate the polysaccharides from the enzyme, which was recovered in the elution step, mainta...
متن کاملCharacterization of Bromelain from Ananas Comosus Agroindustrial Residues Purified by Ethanol Factional Precipitation
Brazil produces over than 70,000 ha of pineapple, being the world leader in pineapple production, and Minas Gerais State alone produces over than 600 kt/y. The pineapple is the marketable plant part, which represents only 23 % of the plant itself, and the remaining plant, stem, leaves, bark and crown, being considered an agroindustrial residue. This residue contains great amount of bromelain, a...
متن کاملCharacterization of Phosphoenolpyruvate Carboxykinase from Pineapple Leaves Ananas comosus (L.) Merr.
Phosphoenolpyruvate carboxykinase has been partially purified from pineapple (Ananas comosus [L.]) leaves. Specific activities obtained show it to be a major activity in this tissue. Above 15 C, the respective activation energies for decarboxylation and carboxylation are 13 and 12 kcal/mol. Below 15 C, there are discontinuities in Arrhenius plots with an associated large increase in activation ...
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ژورنال
عنوان ژورنال: Brazilian Archives of Biology and Technology
سال: 2021
ISSN: ['1678-4324', '1516-8913']
DOI: https://doi.org/10.1590/1678-4324-2021200639